Methods The ciliated cells were isolated by means of mechanical dissociation after papain treatment and a differential interference contrast ( DIC) microscope was used for 1000-fold observation ( objective lens: 100-fold; eye piece: 10-fold). 方法利用木瓜蛋白酶消化及机械分离法分离出豚鼠鼻粘膜单个纤毛细胞,用配备相差或微分干涉相衬(diferentialinterferencecontrast,DIC)装置的倒置显微镜在100倍油镜下(总放大率1000倍)观察。